Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I've seen something similar when using the transmission pathway of the  
microscope (instead of epifluorescence) with high light levels on a  
Rober Cascade 512B EMCCD. Would be interested to know if it is indeed  
a reflection from somewhere near the CCD chip.

John Oreopoulos



Quoting Andrew Resnick <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> All,
>
> This isn't a confocal question per se, but has anyone else had this
> problem (narcissism)? Sometimes our images have a large L-shaped ghost
> which I believe to be the CCD array seeing a reflection of itself.
> This usually happens during low-light level imaging (mostly
> fluorescence methods) and has an intensity around background levels.
> Any hints or tips would be appreciated.
>
> Andy
>
> Andrew Resnick, Ph. D.
> Instructor
> Department of Physiology and Biophysics
> Case Western Reserve University
> 216-368-6899 (V)
> 216-368-4223 (F)