Query posted on behalf of the colleague listed below.

>This is not a confocal question, but more a methodological question. 
>We want to image seizure-related calcium
>fluctuations within rodent cerebral cortical tissue (400um coronal
>slices), using the Fura-2AM dye. Does anyone have experience using this
>dye to capture calcium fluctuations in cortical slice tissue? In
>particular, is there an optimal dye loading technique for this tissue
>which will maximise the calcium signal.

>We would be grateful for any help/suggestions.
>
>We are using a Nikon AZ100 with epifluorescence. Camera is a QuantEM 512SC
>electron multiplying CCD. We have Fura-2 filter set (Semrock
>Brightline); excitation 340nm/380nm; emission 510nm; dichroic
>344-404nm reflection band/415-570nm transmission band.
>
>
>--------------------------------------------------------------
>Dr Logan Voss
>Research Fellow
>Waikato Clinical School
>Hamilton
>New Zealand
>phone: +64 7 8398899 ext8406
>mobile: 021 1629815
"Logan Voss" <[log in to unmask]>


Dr Barry O'Brien
Dept of Biological Sciences,
University of Waikato
Private Bag 3105
HAMILTON
New Zealand

Fax 0064 7 838 4324
Phone 0064 7 838 4179