CONFOCALMICROSCOPY Archives

July 2007

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Kathryn Spencer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 24 Jul 2007 12:21:31 -0700
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Hello all;
	Question of comparison between metal halide illumination and ion
lasers. Can you compare numbers obtained with a power meter at the
objective nosepiece (objective removed)? We took readings from a Prior
Lumen 200 vs. ion and diode lasers. The metal halide was an order of
magnitude brighter (same scope system). The exposure times for each
illuminator were milliseconds. Is there an advantage to the higher
output of the metal halide? Are these photons extra (i.e., is the fluor
saturated and these photons are only contributing to phototoxicity)?
Canyou really compare these two by the numbers?
	Thanks in advance.	
	Kathy

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