Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We are using the Zeiss physiology package daily since 3 years (several
updates). Since we are doing a lot of calcium imaging with different
conventional and confocal systems, we are able to compare different
software packages, including 3rd party software (e.g. ImageJ with
different plugins). We (about 5 happy users) all agree that the Zeiss
physiology package is the best software for calcium imaging and
evaluation. Unfortunately, other formats than the Zeiss *.lsm (like Tiff
series) can not be evaluated as time series.

Regards
Chris

Runions, C.John wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi everyone,
>
> I have recently transferred to a new job and am now having to learn
> new software. We are using a Zeiss LSM 510 to record calcium
> transients in spines of hippocampal neurons.  This type of imaging
> calls for rapid frame scanning or line scanning and the analysis
> software required to get anything useful from the data doesn't seem to
> have come as standard with the system.  From what I understand, we
> need the 'physiology package'.  It is an expensive add-on so I was
> wondering how useful it is.  In other words, is there more to it than
> the one button that is greyed out (measure of intensity across time),
> and are there other software like ImageJ that would do the same for
> much less cash outlay?
>
> Thanks for your help.  John.
>
> _________________________________
>
> C. John Runions Ph.D.
> Ottawa Health Research Institute
> 725 Parkdale Ave.
> Ottawa, Ontario
> Canada
> K1Y 4E9
>
> Phone: (613) 798-5555 ext. 16488
> fax: (613) 761-5024
> email: [log in to unmask] <mailto:[log in to unmask]>
> web: www.ohri.ca <http://www.ohri.ca/>
>
> _________________________________
>


--
Dr. Christian Lohr
Abteilung für Allgemeine Zoologie
Universität Kaiserslautern
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Germany

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