Re Anne McKinney's question:
Does anyone know of a fluorescent dye other than Lucifer Yellow which can
be used for intracellular recording and is fixable?
Anne Ashford and colleagues [[log in to unmask]] have a lot of
experience tracking vacuoles in fungal cells, both in vivo and after
fixation. Relevant papers include Orlovic et al 1993-4 in J Cell Sci.
Dr. Susan Kaminskyj,
Dept. Biological Sciences,
333, Hansen Research Building,
Purdue University,
West Lafayette, IN 47907-1392.
tel (317) 494-4945
fax (317) 496-1496
email [log in to unmask]