CONFOCALMICROSCOPY Archives

December 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
label ?
From:
"Rijstenbil, Jan" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 16 Dec 1997 10:26:00 PST
Content-Type:
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text/plain (17 lines) 
I am looking for a fluorochrome that is an alternative for MBrB of MClB. I
want to label thiols in living cells with a fluorochrome that has to be
excited with visible light (preferably 488nm) and becomes fluorescent after
reacting with the free SH groups.

Other labels (CMFDA/CMEDA) are already fluorescent after reacting with cell
esterases and only "stick" to SH then.

Who can help? Ever heard of OPT in this respect? Thank you for all good
advice.

Jan W Rijstenbil
NIOO CEMO
PO Box 140
NL-4400 AC  Yerseke
Netherlands

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