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Hi,

If you could mail me a print I would sure read it!

Thanks,

Deb Berglund
CBE ERC
MSU
Bozeman, MT  59717

Edward Monosov, Ph.D. wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Deb,
> I've used Brdu to reveal the nascent DNA in the thymine-deprived E.coli.
> cells
> Monosov, E.Z.,  Toukdarian A.,  Helinski D., and  S.J. Singer.(1992)
> Immunoelectron microscopy of deoxyribonucleic acid in procaryotic cells.
> Acta. Histochem. Cytochem. Vol.25, No.1&2, pp 137-141.
> Unfortunately, I don't have the PDF , but,  if you are interested, I can
> Fax you a copy or I can send you a preprint.
> Best.
> Ed
>
> Deb Berglund wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi everybody,
>>
>> Since we are talking about bacteria in flow, I have a new question for
>> you all.   I have a user who wants to do BRDU uptake in bacteria.  She
>> has had success with the procedure in biofilms using confocal and an
>> adapted-from-mammalian labelling prcedure, but when we  rip apart the
>> biofilms and try flow, we seem to be analyzing debris, not individual
>> bacteria.   I have been surprisingly quite successful in looking at
>> bacteria in flow, but think that the BRDU protocol is causing enough
>> cells to lyse/leak that we are seeing labelled DNA clumps.   I tossed a
>> DNA/RNA stain on the samples for threshold and think that we are still
>> seeing non-bacteria debris of varying sizes.
>>
>> Has anyone out there done BRDU uptake in bacteria with flow, and would
>> you send me your protocol?
>>
>> Thanks in advance.
>>
>> Deb Berglund
>> Montana State University
>>
>>
>
> --
> Edward Monosov, Ph.D.
> Director , Cell Imaging & Histology
> The Burnham Institute
> 10901 N. Torrey Pines Rd,
> La Jolla, CA 92037
> Ph:    (858)646-3100 ext. 3206
> Fax: (858)646-3196
> [log in to unmask]
>
>