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Also Anita,

I'm not sure what kind of exposure times you are seeing and where/when
in the stack you start seeing photobleaching but you can also look at a
more sensitive acquisition camera (assuming you are utilizing a CCD
camera) such as the new genre of intensified and/or back-illuminated
CCDs.

You've certainly received some excellent advice for which the research
is sure to keep you busy through the holidays.

Happy Holidays Everyone!

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Stephen Cody
Sent: Wednesday, December 17, 2003 6:04 PM
To: [log in to unmask]
Subject: Re: resolution vs. analysis time

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Dear Anita,

There may be a very simple solution to reduce the time without reducing
the z resolution. However I'm uncertain if it will meet all your
experimental aims. From your email it sounds like you are taking a full
z-series (ie. xyz data set). Would it suit your experimental aims to
collect just a vertical section (xz)? This would be much quicker to
collect and you would end up with just one image per sample, rather than
a z-series. Hence analysis would be a lot quicker.

Stephen H. Cody

Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute For Cancer Research
Post Office Royal Melbourne Hospital
Parkville  Victoria    3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: [log in to unmask]
www.ludwig.edu.au/labs/confocal.html