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October 2020

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Marcia Boulina <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 22 Oct 2020 12:24:55 -0500
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Dear Listserv,
I am looking for the least painful way to handle FLEX files without Columbus. Freshly updated FIJI won't open my files(but i think files are not corrupted, since someone analyzed them with Columbus before). I have forwarded a file to Kevin  and hope to get an answer, but who knows))) Gimp opens the file but image doesnt look right(looks like weird barcode, not cells)+meta is lost. I have requested a trial from Volocity, but Perkin Elmer document on how to convert FLEX to tif in Volocity  is not available on their website. Perkin support was not helpful . I have found couple Py scrips, but they also failed. We do not have OMERO yet. I am happy to hear suggestions and comments))  Thanks in advance!!!

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