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Dear Jeremy,
The Neurotrace fluorescent nissl probes from Molecular Probes label nucleoli, along with any
other RNA in the cell.  You might counterstain with a DNA stain for double segmentation to
potentially avoid the need for a 1:1 relationship of nucleoli to nuclei.

Regards,
Glen
On Tue, 16 Dec 2003, Jeremy Adler wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Any suggestions for fluorophores that will highlight nucleoli in fixed
> and live tissue ?.
>
> assuming a 1:1 relationship of nucleoli to nuclei it should be easier to
> count nucleoli since they are smaller and less likely
> to appear to touch, which then creates problems for automated detection.
>
> Jeremy Adler
> CIAL
>