CONFOCALMICROSCOPY Archives

March 2004

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Subject:
From:
Daniel Mackay <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 4 Mar 2004 14:58:21 +0000
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sorry, should have read:

".....I'm curious as to why one would want
to use 12 bit pixel depth  when capturing images at 1024X1024 *pixels*."

At 02:47 PM 3/4/04 +0000, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi,
>
>Some advice please.
>
>As a relative "newbie" to this game, I'm curious as to why one would want
>to use 12 bit pixel depth  when capturing images at 1024X1024 images. This
>would give over 4000 grey scales as opposed to 255 (8 bit). Isn't 255 enough?
>
>Here are some comments I received wrt some images I recently produced.
>These were made for metamorph quantification, not necessarily for high
>resolution.
>
>Only 8 bit pixel depth (256 gray scales) imaging used, not
>appropriate with 1024X1024 image sizes, which defeats the purpose
>acquiring high of resolution images from these large scans.
>  Should use 12 bit imaging  as minimum with frame scan (can use 12
>bit 1024x1024 for images to be printed.
>
>
>
>??????
>
>
>Danny.
>

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