Dear all,

Sorry I mixed up the dye names in my previous posting.

It was not "7-Actinomycin D" but its derivative, "7-Aminoactinomycin D".
The former is not fluorescent.


> Dear Steffen,
>
> Yes, Chronomycin A3 works fine in fixed cells as a green fluorescent
> DNA dye.  On the other hand, 7-Actinomycin D, available from Sigma or
> Molecular Probes, is a good choice for red staining of the nucleus.
> It binds selectively to GC pair in DNA,
> and you'll get NO specific fluorescence in RNA-containing structures,
> - a lot easier than propidium iodide or any other Feulgen dyes.
>
> Tets


***************************************
Dr Tetsuhiro Minamikawa
Dept. Biochem & Mol Biol
Monash University
Wellington Road, Clayton, Victoria 3168
Australia
Ph: +61 3 9905 1430
Fax: +61 3 9905 4699
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