The RI of my mounting media, which contains 8.5% of Mowiol 4-88, is
1.41 at 22 degrees Celsius. Hope this helps.
>If no one has an answer, send me some and I will run it on a refractometer,
>temperature controlled, etc. This won't give you the delta t but I could
>run a few different temperatures around what you plan to use.
>
>
>
>At 03:33 PM 1/4/01 -0600, Tom Phillips wrote:
>>I would like to expand Susan's question to ask if anyone knows the RI
>>of Mowiol? Even better, is there a reference or web site that lists
>>the RI of common commercial and homemade mounting media. Thanks, tom
>>
>>
>>
>>
>>>Dear Confocalists,
>>>
>>>I would like to test the mounting medium called Permafluor. It is made by
>>>Shannon Lipshaw, marketed by Immunotech in Europe, and available in the
>>>U.S. from Beckman-Coulter, product #IM0752. B-C has not been able to get a
>>>response, from the developer, to my questions . Does anyone know what the
>>>refrative index of Permafluor is? How does it compare to Prolong Antifade?
>>>Has anyone used the current formulation to mount thick (350 micron) tissue
>>>samples? We're looking at fixed, whole cricket ganglia.
>>>Thank you,
>>>
>>>Susan Krall Krueger
>>>Center for Computational Biology
>>>Montana State University
>>>Bozeman, MT 59717
>>>406-994-7748
>>--
>>Thomas E. Phillips, Ph.D.
>>Associate Professor of Biological Sciences
>>Director, Molecular Cytology Core Facility
>>
>>3 Tucker Hall
>>Division of Biological Sciences
>>University of Missouri
>>Columbia, MO 65211-7400
>>(573)-882-4712 (voice)
>>(573)-882-0123 (fax)
>
>Damian Neuberger, Ph.D.
>Baxter Healthcare, Inc.
>WG3-2S
>P.O. Box 490
>Round Lake, IL 60073
>e-mail: [log in to unmask],
>Tel: 847-270-5888
>Fax: 847-270-5897
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