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We've found prolong to be quite sensitive to storage, with it not setting properly after it's been in the fridge for a while (a week or two). The solution seems to be to aliquot and freeze on opening the bottle.

You also don't want to have too much excess moisture on the slide before mounting.

Cheers,
David


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On Tue, Jul 3, 2012 5:36 PM NZST Kelvin Poon wrote:

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>Although the protocols say that 24 hours is sufficient for solidification, our 
>microsphere beads appear to swim around even after two full days at room 
>temperature. Does anybody have a better estimate for complete curing or when 
>it would be safe to seal with nail polish?