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Dear Friends
I would like to inform you that we edited a book on FRET
Microscopy (see below the Table of Contents). Most of the
authors are the teaching Faculty of annual workshop on
FRET Microscopy, March 8-12, 2005
(www.kcci.virginia.edu).
The book is 'In Press' and we expect this book will be
published during or around March 2005.
Best wishes,
ammasi
-----------------------
(Expected March 2005; Oxford University Press;
www.oupusa.org)
MOLECULAR IMAGING: FRET MICROSCOPY AND SPECTROSCOPY
EDITORS: AMMASI PERIASAMY AND RICHARD N. DAY
University of Virginia, Charlottesville, USA
Table of Contents
Preface
Cover page comments ? Thomas Jovin, Germany
1. Proteins and the Flow of Information in Cellular
Function
Robert Kretsinger
2. Basics of Fluorescence and FRET
Zygmunt Gryczynski, Ignaci Gryczynski and Joseph
Lakowicz
3. An Introducrion to Filters and Mirrors for FRET
Michael Stanley
4. FRET Imaging in the Wide-field Microscope
Fred Schaufele, Ignacio Demarco and Richard N. Day
5. Confocal FRET Microscopy - Study of Clustered
Distribution of Receptor-ligand Complexes in Endocytic
Membranes
Horst Wallrabe and Margarida Barroso
6. Multiphoton FRET Microscopy for Protein Localization in
Tissue
James D. Mills, James R. Stone, David O. Okonkwo, Ammasi
Periasamy and Gregory A. Helm
7. FRET data analysis ? the Algorithm
Ye Chen, Masilamani Elangovan, and Ammasi Periasamy
8. Photobleaching FRET Microscopy
Anne Kenworthy
9. Single-Molecule FRET
Sungchul Hohng and Taekjip Ha
10. FRET Measurements using Multispectral Imaging
Mary Dickinson and Scott Fraser, Caltech
11. Real-time Fluorescence Lifetime Imaging and FRET using
Fast Gated Image Intensifiers
Glen Redford and Robert M. Clegg
12. Streak FLIM: A Novel Technology for Quantitative FRET
Imaging
V. Krishnan Ramanujan, Jian-Hua Zhang, Victoria. E.
Centonze and Brian Herman
13. Time-Correlated Single Photon Counting (TCSPC)
FLIM-FRET Microscopy for Protein Localization
Ye Chen and Ammasi Periasamy
14. Bioluminescence RET (BRET): Techniques and Potential
Mohammed Soutto, Yao Xu, and Carl H. Johnson
15. Quantifying Molecular Interactions with Fluorescence
Correlation Spectrosocpy
Keith Berland
16. Mapping Molecular Interactions and Transport in Cell
Membranes by Image Correlation Spectroscopy
Elenora Keating, Claire M. Brown and Nils O. Petersen
--------------------
Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http//:www.kcci.virginia.edu
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