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Hello to all,

We recently began using the Cargille 37 oil with the temperature chamber on the
microscope.  I found significant fluorescence from the oil when exposed to the
HBO lamp (I could not see the eYFP because of the blue background).

So I used the 20x air objective and I was able to obtain an image from the
fluorescence from a drop of 37 oil placed on a coverslip as a sample
(excitation at 488 or 458nm and pinhole at the usual confocal setting).

Is this the norm?

Regards,
Sophie
_______________________________________
Sophie M.K. Brunet, Ph.D.
Research Officer
Optical Spectroscopist, Laser Systems and Applications
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306-966-1719 (office)   306-966-1702 (fax)
_______________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
Thorvaldson Bldg.
110 Science Place
Saskatoon, SK   S7N 5C9