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Hello Sarah,

Huygens as far as I know does not do any '2D' deconvolution, you might want
to use one of the filters provided by Huygens though. For these it is not
necessary to provide a PSF.

The problem you experience with the contribution of the leave structure
might be addressable by a spectral un-mixing approach.

Cheers, jens


Sarah Kefayati wrote:
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello all:
> 
> I am collecting in situ psfs from Arabdiopsis leaves.For me it is
> impossible to collect PSF's in z direction  as I will also capture the
> leaf structure too and I only collect data in XY plane.
> I am wondering if it is possible to do 2D deconvolution by these
> collected PSF s with Huygens software(we haven't get the soft ware yet
> we would like to try this with the test licence of the software).
> 
> I really appreciate any information and any idea.
> 
> Sarah
> 
> 

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