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Dear microscopists,
I use ImageJ StackReg. plugin for time series confocal image alignement  
(Rigid body mode). This plugin works like this:
"StackReg. This JAVA class for ImageJ performs the recursive registration  
(alignment) of a stack of images, so that each slice acts as template for  
the next one".
My problem is that if each slice acts as a template for the next one and  
if i have 2000image stack, there is always slice shift between the first  
and the last images. My question is - do you know about any plugin which  
use as the template always the first image?
Thank you

-- 
David Sojka
Institute of Physiology
Academy of Sciences of the Czech Republic
Videnska 1083
142 20 Praha 4
e-mail: [log in to unmask]
tel:+420775901930