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Hello all

We at the Cellular Imaging Core also recently tried the STEDYCON and our
experience was that it proves to be a notable addition to upgrade a WF
microscope.

I do agree with it being restricted-- I think STEDYCON has a way to go with
bringing the confocal imaging bit up to competitive standards (& I've been
vocal on that) but the STED images produced were a lovely entryway for
smaller labs and cores to dip their toe into super-resolution. I am
currently considering it being placed at an empty port on our spinning disk
confocal or even on an old Olympus body (it has a DSU which I may
cannibalize). However, since we are a fairly large core facility, I'm also
looking deeply at their Expert Line (& I will keep an eye out for
photoconversion of the dyes!)

Lastly, service is always a huge 'Yay or Nay' point on whether or not to
buy an instrument but I personally am hopeful that they will be up to the
task. They've been exceptionally responsive to us thus far and I have
reason to believe they will continue to be in responsive the future.

It is my opinion that industry giants have an advantage in service packages
but that start-up and mid-age companies have a huge advantage when it comes
to niche capabilities... so I am eager to see what they will do.

Cordially,
cvic

***NO COMMERCIAL INTEREST***



On Wed, May 23, 2018 at 10:40 AM, Alison North <[log in to unmask]>
wrote:

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> *****
>
> Hi Stan,
>
> Fortunately we had a very different experience to Christian last year when
> they brought the STEDYCON to the OMIBS course at Woods Hole.   Karsten had
> it up and running and working perfectly within 1 hour of unpacking the
> boxes, and we were collecting fabulous images that same evening.  I agree
> with the comments about having to use appropriate far red and red dyes for
> the 775 nm laser - we used STAR Red and Alexa 594 - and I also agree that
> the confocal images aren't as good as on a regular confocal system, but
> that didn't worry me too much as I would never intend to use it for that
> purpose.  I can't comment on the photoconversion question as I must admit I
> didn't look for that and we were only using a few samples as we had limited
> time.
>
> We also had the full blown STED system on demo in my lab for around 3
> weeks in early 2017, and I found their service to be very good - they were
> very helpful in trying to fix any problems.
>
> I guess this is typical of demos - it always seems to be the case that one
> goes well and another not so well...
>
> All the best,
>
> Alison
>
>
>
> On 5/23/2018 8:40 AM, Christian Feldhaus wrote:
>
>> *****
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>> Post images on https://urldefense.proofpoint.
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>> in your posting.
>> *****
>>
>> Hi,
>>
>> we had a Stedycon demo end of last year for 3 weeks. We could only test
>> it for 1 week as abberior couldn't get it into working condition for 2
>> weeks. We have been very disappointed with the quality of their service.
>>
>> As Josef already mentioned the device is quite restricted in what it can
>> do. Furthermore, we observed photoconversion of the STAR RED dye which can
>> make interpreting your results quite an adventure.
>>
>> If you want more details about our test please feel free to contact me
>> off list.
>>
>> Best,
>>
>> Christian
>>
>>
>> On 22.05.2018 23:34, Stanislav Vitha wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
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>>> n.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=JeTk
>>> UgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNm
>>> FbqYvikvIZS5ns3-USwvMOuLo&m=FMLs94PJJntUQiT6at158OvdNgwH69KU
>>> Qte2PgcWlaA&s=FS7K1KlwPKnu_AXGGZbhRU6wl70E2Y5HIZGBE1C9rb4&e=
>>> Post images on https://urldefense.proofpoint.
>>> com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=JeTkUgVztGMmh
>>> KYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvI
>>> ZS5ns3-USwvMOuLo&m=FMLs94PJJntUQiT6at158OvdNgwH69KUQte2PgcWl
>>> aA&s=1SLqVtaDFRA2LLh2nu-mQ1Iq5sleW9rD5CgYRFDgC2o&e= and include the
>>> link in your posting.
>>> *****
>>>
>>> Howdy,
>>>
>>> we are considering the Abberior Instruments STEDYCON shoebox STED system
>>> as an entry into superresolution for our core facility. We have not had the
>>> opportunity to see it in action yet and would appreciate your
>>> feedback/comments if you have used it or are using it currently.
>>>
>>>   Thank you in advance!
>>>
>>> Best Regards,
>>>
>>> Stan Vitha
>>> Microscopy and Imaging Center
>>> Texas A&M University
>>>
>>
>>
> --
> Alison J. North, Ph.D.,
> Research Associate Professor and
> Senior Director of the Bio-Imaging Resource Center,
> The Rockefeller University,
> 1230 York Avenue,
> New York,
> NY 10065.
> Tel: office     ++ 212 327 7488
> Tel: lab        ++ 212 327 7486
> Fax:            ++ 212 327 7489
>