***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Hi Michael 561nm? Which light source are you using? I wonder if the stripes could be explained by some unstability in the light source. Med vanliga hälsningar/Best regards Sylvie Le Guyader | PhD Live Cell Imaging core facility manager Bionut | Karolinska Institutet Hälsovägen 7C | 14157 Huddinge +46 73 733 5008 [log in to unmask]<mailto:[log in to unmask]> | LCI website<https://ki.se/en/bionut/live-cell-imaging-core-facility-lci> | LCI blog<http://microscopykarolinska.se/> Karolinska Institutet – a medical university ________________________________ From: Confocal Microscopy List <[log in to unmask]> on behalf of Cammer, Michael <[log in to unmask]> Sent: Saturday, December 30, 2023 4:07:07 AM To: [log in to unmask] <[log in to unmask]> Subject: horizontal stripes question with spinning disk [Some people who received this message don't often get email from [log in to unmask] Learn why this is important at https://aka.ms/LearnAboutSenderIdentification ] ***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FSUBED1%3Dconfocalmicroscopy%26A%3D1&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=FvzMdm%2FUuRYZOcRvsEWOOYs6LGwgsp5xsW1p51nNNic%3D&reserved=0<https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1> Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=meSPLHAhUrtHq0Ioy6lN9ATneHxTErmI9QznAcpwcP4%3D&reserved=0<http://www.imgur.com/> and include the link in your posting. ***** Happy New Year! We are curious about an issue we have with a Crest V3 spinning disk confocal with a Kinetix camera. We have horizontal bands on our images in the 400-600 range of intensities in the 16 bit dynamic range mode. This happens with all lenses. We are curious why does this happen? Is this it normal? An example image is at https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fmicroscopynotes.com%2Ftemp%2FKinetixSpinningDiskStreaks2023%2Fscreensnap.png&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NkypgiTowxfwy2fjU%2BGpoz0JSRjYsYMJXJ7WOZZ%2FSRU%3D&reserved=0<https://microscopynotes.com/temp/KinetixSpinningDiskStreaks2023/screensnap.png> The image was collected with a 561 nm laser and a standard narrow band red emitter. The camera was set to 2 x 2 binning but the results look essentially the same with no binning. The stripes remain in a fixed location and don't change with exposure time. I also posted the full res version https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fmicroscopynotes.com%2Ftemp%2FKinetixSpinningDiskStreaks2023%2Ffull_res.tif&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0xWanTS8KKcQHIlIzdUG%2BERTmSRcjD4ldZiKeiI9FRs%3D&reserved=0<https://microscopynotes.com/temp/KinetixSpinningDiskStreaks2023/full_res.tif> Best regards- Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, New York, NY 10016 [log in to unmask]<mailto:[log in to unmask]> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fnyulmc.org%2Fmicros&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=P%2Fxc2XS3EjgORkVMAec2BdqK7ByEg1xemRMfP75lYQE%3D&reserved=0<http://nyulmc.org/micros> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fmicroscopynotes.com%2F&data=05%7C02%7Csylvie.le.guyader%40KI.SE%7C7b3161400b2f49ccd77008dc08e4ab84%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638395025440714807%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=lKIOcllxuKAPSMW1sm7uHVkqkjtIi%2FplbofuJgyzvWc%3D&reserved=0<http://microscopynotes.com/> Cell voice (no text) 1-914-309-3270 Office 1-646-501-0567 När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. 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