*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Sunando
Not sure why you need only the spinning disk based FLIM system to image FLIM-FRET in live cells.
We use and train in the FRET workshop both photon counting and camera based FLIM system for live cells. Camera based system is frequency domain, wide-field microscope and photon counting mode is time domain. You can use two-photon based system for photon counting or confocal based photon counting system. Becker & Hickl, Germany, sells stand alone confocal FLIM-FRET system for very reasonable price.

If you would like to explore and get trained on the FLIM-FRET system attend our workshop. Few companies system will be used to train the workshop participants including Becker & Hickl, Lambert instruments and ISS. 
The workshop is during March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
Hope this helps.

Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
(A University Imaging Center)
Biology, University of Virginia
Mail or FedEx: 485 McCormick Rd.
Charlottesville, VA 22904.
Office Location: Physical Life Sciences Building (B005)
90, Geldard Drive, Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of samuel connell
Sent: Saturday, February 16, 2013 11:38 AM
To: [log in to unmask]
Subject: Re: Live cell set up with FLIM

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Commercial Response:

Hi Sunando,

As George mentioned, Akira Chiba and others have set up systems with Intelligent Imaging Innovations (3i) to execute spinning disc confocal, wide-field, or TIRF FLIM systems. Importantly as your suggested, all three of these experimental paradigms are camera based. 3i is currently selling a frequency domain FLIM camera, packaged with our SlideBook software and LaserStack, with significant improvements in both QE and resolution compared to the previous industry standard. If you are interested in a discussion on how this approach would fit into your department's needs, feel free to contact me off list.

FYI: 3i also delivers TCSPC FLIM systems in conjunction with our 2-Photon system offerings.

Kindest Regards,
--
Sam

------------------------

Samuel A. Connell

Senior Applications Scientist

Intelligent Imaging Innovations, Inc

3250 Ocean Park Blvd, Suite 202

Santa Monica, CA  90405

Office: (303) 607-9429 x6926

Cell:    (858) 692-4510

[log in to unmask]

www.intelligent-imaging.com


On Sat, Feb 16, 2013 at 6:36 AM, George McNamara
<[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.u
> mn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> )See:
>
> Buranachai C, Kamiyama D, Chiba A, Williams BD, Clegg RM. Rapid 
> frequency-domain FLIM spinning disk confocal microscope:
> lifetime resolution, image improvement and wavelet analysis. J Fluoresc.
> 2008 Sep;18(5):929-42.
> doi: 10.1007/s10895-008-0332-3. PubMed PMID: 18324453.
>
> Akira Chiba here at UMiami has two spinning disk FLIM microscopes (see 
> above for details or contact Akira). See also 
> http://www.miami.edu/index.**php/features/propelling_**proteomics-1/<h
> ttp://www.miami.edu/index.php/features/propelling_proteomics-1/>
> http://ispinproject.org/
>
> If you cannot afford a Yokogawa spinning disk, check out the X-Light 
> at http://www.biovis.com/x-light.**htm 
> <http://www.biovis.com/x-light.htm>
>
>
> If you are using very thin cells (low autofluorescence also good - see 
> PubMed 23285248), non-fluorescent culture media, then spinning disk 
> (or DMD or similar multi-point) not needed. See also Tom Jovin's PAM 
> scope, which can do FLIM in widefield or optical sectioning modes:
>
> Hanley QS, Lidke KA, Heintzmann R, Arndt-Jovin DJ, Jovin TM. 
> Fluorescence lifetime imaging in an optically sectioning programmable 
> array microscope (PAM). Cytometry A. 2005 Oct;67(2):112-8.
> PubMed PMID: 16163693.
>
> Tom also has nice reviews on FRET:
>
> Jares-Erijman EA, Jovin TM. Imaging molecular interactions in living 
> cells by FRET microscopy. Curr Opin Chem Biol. 2006 Oct;10(5):409-16. 
> Epub 2006 Sep 1.
> Review. PubMed PMID: 16949332.
>
> Jares-Erijman EA, Jovin TM. FRET imaging. Nat Biotechnol. 2003 
> Nov;21(11):1387-95. Review. PubMed PMID: 14595367.
>
>
>
> Enjoy,
>
> George
>
>
> On 2/15/2013 12:13 PM, sunando wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.
>> umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Hello All
>> We are intending to set a live cell imaging set up at our department. 
>> I am interested to carry out FRET/FLIM studies.
>> Would someone help me in suggesting about the camera ?
>>
>> We would go for Epi set up. Does not funding for confocal....
>>
>> Thank you advance
>> Sunando
>>
>>
>>