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At 17:34 17.12.2003, you wrote:
>For this
>reason water objectives often give the best performance possible
>because the RI mismatch is considerably less.

Just a remark on the side: I'm not sure what the RI of a bacterial biofilm
is but I think it might be closer to glycerol or even oil than to water, so
using a glycerol or oil objective might actually give you better results
than a water objective. I'd make sure to test this before spending money on
a new objective.

And yes, IMHO, opening the pinhole would be the best way to allow
increasing the Z-step width w/o running into problems with undersampling.

Just my $0.02, for what they're worth,

Beat