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I do not know much about this incubator system however does it use
tubing at any end/s? You might want to check what the tubing material is
composed of and their rate of gas exchange. The Cole Parmer catalogues
contain a lot of information on this subject, it may be worth a look if
you think this could be a problem.
It may also be an idea to fit some sort of gas sensor in the room, just
from an OH&S point of view to monitor the CO2 levels or monitor other
gas levels in the room that may be used. It may save you or somebody
else from a potential disaster!
Deanne Catmull
Research Assistant
School of Dental Science
University of Melbourne
720 Swanston St
Carlton 3010
Fax: 9341-1597
Ph: 9341-1577
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Guy Cox
Sent: Monday, February 25, 2008 10:02 AM
To: [log in to unmask]
Subject: Re: [SPAM] CellCubator climate chamber question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Excess CO2 in the room does not make you fall asleep and never
wake up - you start hyperventilating and get a splitting headache.
It's oxygen depletion that's dangerous - that is symptomless and
fatal. You should be more concerned about the dry nitrogen passing
through your TiS laser than the CO2 in your incubator! (And, of
course, even more careful about liquid nitrogen).
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net <http://www.guycox.net/>
________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Rietdorf, Jens
Sent: Monday, 25 February 2008 7:39 AM
To: [log in to unmask]
Subject: Re: [SPAM] CellCubator climate chamber question
Search the CONFOCAL archive at
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Dear Herman,
when we were constructing an incubator at embl, we calculated the CO2
loss at 5% to be less than the CO2 produced by the operator in front of
the device. I admit, we didnt have Al in mind, but the fact that the
student inside the tiny little microscope room could fall asleep and
never wake up again, but we measured and thats what we found.
regards, jens
---
Dr. Jens Rietdorf <mailto:[log in to unmask]>
Head Microscopy
Novartis Research Foundation
Friedrich-Miescher-Institute <http://www.fmi.ch/> , wro1066.2.16
Maulbeerstr.66, CH-4058 Basel, Switzerland
phone +41(61)69-75172 mobil +41 798284737
Email:rietdorf(at)fmi.ch
________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Herman Favoreel
Sent: Donnerstag, 21. Februar 2008 16:06
To: [log in to unmask]
Subject: [SPAM] CellCubator climate chamber question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,
My apologies for this non-confocal related question. For live cell
imaging experiments, we use a CellCubator climate chamber. When we put
the CO2 level to 5%, and then close the CO2 faucet but keep the
airstream going, which is supposed to be a closed loop, the CO2 level
drops to below 1% within 10 to 15 minutes.
That seems very fast to me and appears to point to quite a high CO2
consumption, and (with Al Gore in mind :) ) it makes me reluctant of
using 5% CO2 conditions for long term experiments (e.g. several days). I
wonder whether this is a normal speed of CO2 loss, could anyone share
his or her experience with the CellCubator or other incubators and their
CO2 consumption ?
Many thanks in advance,
Herman
Herman Favoreel
Department of Virology, Parasitology, and Immunology
Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133
9820 Merelbeke
BELGIUM
Phone: ++ 32 9 264 73 74
Fax: ++ 32 9 264 74 95
E-mail: [log in to unmask]
website: http://www.vpi.ugent.be
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