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We have a Zeiss 510 Meta, and would like to learn more its software too. However, the Zeiss FTP site listed below requires an Username and Password. How can we access the FTP site?
Shaohui Huang, Ph.D.
Institute for Environmental Medicine
UPenn School of Medicine
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Jacqui Ross
Sent: Wednesday, July 27, 2011 8:56 PM
To: [log in to unmask]
Subject: Re: Settings adjustment with depth for a Zeiss LSM 510
Hi Pedro,
We also have an LSM 510 META. If you have the Guided Tour PDF, page 45 explains how to use the Auto Z Brightness correction. However, you can only set 2 positions (with associated gain/offset) so sometimes this isn't enough.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND
Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484
http://www.fmhs.auckland.ac.nz/sms/biru/
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Pedro Almada
Sent: Wednesday, 27 July 2011 10:25 p.m.
To: [log in to unmask]
Subject: Re: Settings adjustment with depth for a Zeiss LSM 510
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Hi Esteban and Martin,
Thanks alot for the help. I admit I wasn't aware of the option being in the software itself. I'll try and have a look at their preparations myself and check for oversaturation (if I remember their pictures correctly, that sounds like a definite possibility).
Thanks again, this is a very helpful and interesting list.
All the best,
Pedro
On 26 July 2011 16:37, G. Esteban Fernandez
<[log in to unmask]>wrote:
> The acquisition software (LSM or ZEN) has that function built in, no
> need for a macro. It is called something like "Z brightness
> correction" and is in the Z stack panel.
>
> Software manuals for LSM and ZEN can be found at the Zeiss FTP site here:
> ftp://lsm.zeiss.com/LSM/User_Area/Manuals/
>
> -Esteban
>
> On Tue, Jul 26, 2011 at 7:26 AM, Pedro Almada <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear listers,
>>
>> Does anyone know of a VBA macro for the Zeiss Meta which allows users
>> to adjust settings such as gain and laser power according to current
>> focus for the Zeiss LSM 510? I am interested in developing this
>> myself but if there is something already made, that would be great...
>>
>> If you are curious what it's for, it's for some of our users who
>> image whole drosophila brains for structural information. Their
>> common complaint is that half-way through their z-stack they loose
>> image brightness until the last slices are barely visible. Clearing
>> methods like Methyl salicilate aren't really adequate for neuronal
>> structure I'm told, which leads me to consider this method.
>>
>> Either way, any information is welcome.
>>
>> Thanks for reading,
>> Cheers,
>> Pedro Almada
>> *
>> *
>> Research and Microscopy Technician
>> Unidade de Imagiologia Celular,
>> *Instituto Gulbenkian de Ciência*
>> Rua da Quinta Grande, 6
>> 2780-156 Oeiras
>>
>> Phone: + 351 214 464 607
>> Ext: 607
>>
>
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