You may want to look at our recent paper:
Van Steensel, B., Van Binnendijk, E.P., Hornsby, C.D., Van der Voort,
H.T.M., Krozowski, Z.S., De Kloet, E.R. and Van Driel, R. (1996). Partial
colocalization of glucocorticoid and mineralocorticoid receptors in discrete
compartments in nuclei of rat hippocampus neurons. J. Cell Sci. 109, 787-792.
Best regards,
Roel van Driel
>Hello Confocalists
>
>We are becoming more and more interested in determining quantitatively the
>relationships between different classes of objects in three dimensional
>space, as determined by 3D reconstructions of either confocal or
>conventional fluorescence microscopy images. I am familiar with many areas
>of statistics and stereology, as well as the newer methods for assessing
>the true sizes and distributions of objects (disector type methods etc).
>However, it seems that none of these methods by themselves really let you
>determine whether an observed distribution of objects in 3D space is
>random, clumped, or significantly associated with another class of objects.
>I think this problem has been tackled to some degree by ecologists who need
>to determine similar relationships betweeen organisms in forests etc, and
>is based on various forms of nearest neighbour and / or cluster analysis. I
>am more than happy to delve into this literature more carefully, and, if
>necessary, try to develop some more appropriate methods. But before I
>start, I am just wondering if anyone out there in microscopy land knows of,
>or has used, this sort of statistical approach?
>
>I should emphasise that this question is independent of the problems
>associated with the accuracy of detection, the validity of the
>reconstructions etc - although obviously the end result depends on how well
>you can do those steps and, more important, how well you can estimate the
>errors associated with them...
>
>Thanks in advance,
>IAN
>Professor Ian Gibbins
>Department of Anatomy and Histology
>Flinders University of South Australia
>Phone: +61-8-2045271
>FAX: +61-8-2770085
>e-mail: [log in to unmask]
>
>
Roel van Driel
E.C. Slater Institute
University of Amsterdam
Plantage Muidergracht 12
1018 TV Amsterdam
The Netherlands
e-mail: [log in to unmask]
fax: +31.20.525.5124
phone: +31.20.525.5150
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