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Hi Everyone,

I use coverglass from Carolina Biological Supply for my primary neuron cultures, coated in poly-L-lysine. According to their site, it’s the "the famous Deutsche Spiegelglas.” Maybe the D stands for Germany :) ! Good luck! https://www.carolina.com/catalog/detail.jsp?prodId=633037&s_cid=ppc_gl_products&utm_source=google&utm_medium=cpc&scid=scplp633037&sc_intid=633037&gclid=CjwKCAjwnrjrBRAMEiwAXsCc48U2hkpOztMo9SuwpAQWeg1rqXsF8Hye8tA_TgnKua9gIfHuswOSBhoCFEIQAvD_BwE

Sara Parker
Postdoctoral Fellow, Mouneimne Lab
(520) 626-3860
[log in to unmask]
Department of Cellular & Molecular Medicine
University of Arizona
On Jan 20, 2020, 2:09 PM -0700, Julia Edgar <[log in to unmask]>, wrote:
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Hi Claire
I can't answer you actual question, but this information might still be helpful.
In my experience, neurons adhere much better to glass coverslips coated with poly-L-lysine in boric acid buffer versus poly-L-lysine in water. The difference is remarkable.
Best wishes
Julia

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Claire Brown, Dr.
Sent: 20 January 2020 20:59
To: [log in to unmask]
Subject: Coverslips for Primary Neuronal Cultures

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A colleague of mine asked me this but I'm not familiar with "D" Coverslips.

I thought someone here might know.

For microscopy with our primary neuron cultures, we have successfully used "D" coverslips from Fisher (12-CIR-1D, #12-545-82) for at least a decade now, but the product is now discontinued. Historically, D coverslips have been recommended for neurons because neurons adhere and grow better on them.
Am I correct that the "D" refers to a particular type of German glass, Schott D263M? I've found that Electron Microscopy Sciences, Ted Pella, and Thorlabs seem to sell coverslips made with Schott 263M glass. Or does "D" refer to "desag", in that case I'm not sure I've found appropriate equivalents.
If you have any recommendations and insights, or other suppliers to consider, I'm all ears.


Sincerely,

Claire