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Dear Maryse
We and others have used quantum dot nanocrystals for imaging fixed and live
cells. The nanocrystals are chemically and photochemically stable,
especially for the application you describe. There are no apparent effects
on cell function. They can be used to label the cell surface or can be
introduced into cells in a number of ways. The technology and cell and
in-vivo applications are described in:
Wu X., et al. Immunofluorescent labeling of cancer marker Her2 and other
cellular targets with semiconductor quantum dots. Nature Biotechnology
Volume 21 No.1 pp41-46
Dubertret B., et al. In vivo imaging of quantum dots encapsulated in
phospholipid micelles. Science Vol 298:1759-1762 (Nov 29, 2002).
Jaiswal, J. et al. Long term multiple color imaging of live cells using
quantum dot bioconjugates. Nature Biotechnology online Dec 2, 2002 doi:
10.1038/nbt767.
Åkerman et al. Nanocrystal Targeting In Vivo. Proc. Nat'l Acad. Sci. USA
99:12617-12621 (2002).
These products are available from our website www.qdots.com. We solicit
your (and others!) input as to how to improve these products for your
specific needs.
Best regards
Andy Watson
-----Original Message-----
From: Maryse Bailly [mailto:[log in to unmask]]
Sent: Monday, February 10, 2003 2:50 AM
To: [log in to unmask]
Subject: cell volume and/or membrane marker
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear all,
We are trying to image human fibroblasts live in
collagen gels. While we have no problem with other
types of cells, these fibroblasts are so huge that our
standard marker (GFP) bleaches so fast that by the
time we have gone through the whole cell volume, there
is almost no fluorescence left (let alone for doing a time-lapse...). We
have used both confocal and standard fluo/CCD imaging but even after all
tricks tried (reducing the number of slices, of time points, minimal light
exposure...), we are still limited to 2 or 3 times the cell volume before
the fluo dies. We have tried using a standard cell dye CFDA-SE, but with no
better results. Would any one have any ideas on how to label these big
cells (whole volume or at least
membrane) with a more "unbleachable" approach?
Cheers
Maryse
=====
Maryse Bailly, Ph.D
Division of Cell Biology, Institute of Ophthalmology
University College London
11-43 Bath Street, London, EC1V 9EL
Tel: (44) 20 7608 4051
Fax: (44) 20 7608 4034
Email: [log in to unmask]
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