I usually use those fluorescent plastic slides which Chroma
give away at conferences.  They seem at least as reproducible
as a dye solution and much more convenient.  There is a range of
colours so you can pick one which more or less matches the 
fluorochrome you are using.

                                                    Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09, 
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of MODEL, MICHAEL
Sent: Friday, 5 December 2008 8:49 AM
To: [log in to unmask]
Subject: Re: Presenting Semi-quantitative Data

Maria - I use a fluorescent field made of a concentrated (10% or so)
dye. Then you simply divide your image by the image of a dye and get the
intensities. I think it's easier than to measure beads.

Mike Model


Michael Model, Ph.D.
Confocal Microscopy,
Dpt Biological Sciences,
1275 University Esplanade,
Kent State University, Kent, OH 44242
tel. 330-672-2874



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Maria Mazzillo
Sent: Thursday, December 04, 2008 3:40 PM
To: [log in to unmask]
Subject: Presenting Semi-quantitative Data

Hello,

I have taken photos using fluorescent beads and am using ImageJ to
compare
the fluorescence in the sample to the beads.  I have been looking at
others
who have presented semi-quantitative data but there is a huge variation
in
how it was presented.  I was wondering if anyone has done this kind of
thing
before and what the best way is to present the data (graph, table,
etc.).

Thanks,

Maria Mazzillo
Auburn University