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for registering serial sections of mouse brains we sometimes poke 
holes in the brains before slicing.


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello All,
>
>We're taking some multimodal images of skin (SHG, 2P (auto), 
>reflectance, and Raman), 20-60x.
>
>The SHG, 2P, and reflectance are on one scope and the Raman is on 
>another (pretty cool images in all modes).
>
>We've reached a quandary on how to register these images (even get 
>near similar fields of view, which are between 50-250 um).
>
>A pen mark would be way too big.  Too many hair follicles to narrow 
>down to.  Latex beads will probably float away (the skin needs to 
>stay hydrated).  A grid coverslip? Maybe a gene gun?
>
>The scopes are in different buildings, so stability of the "mark" IS an issue.
>
>Any and all input is appreciated, again.
>Best,
>
>Gary
>
>
>
>Gary Laevsky, Ph.D.
>Keck Facility Manager, CenSSIS
>Northeastern University
>302 Stearns
>360 Huntington Ave.
>Boston, MA 02115
>Office(617) 373 - 2589
>Lab(617) 373 - 7756
>Fax(617) 373 - 7783
>
>http://www.censsis.neu.edu
>
>http://www.ece.neu.edu/groups/osl
>
>http://www.keck3dfm.neu.edu

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
URL:  http://www.aecom.yu.edu/aif/