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Dear Ann,
Have you checked those plates?
https://www.appletonwoods.co.uk/product/96-well-spheroid-microplate-blk-clr-round-btm-with-lid-ula-sterile-indiv-wrapped-corning/
I’ve never used them but maybe this is what you are looking for?
Best,
Valentina
Dr Valentina Lo Schiavo
Senior Application Specialist
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On 2 Jun 2023, at 10:01, Steffen Dietzel <[log in to unmask]<mailto:[log in to unmask]>> wrote:
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Dear Ann,
since there do not seem to be a lot of answers, maybe this thought: have you thought of filling a drop of gel into the wells? The capillary forces at the edge of the well should draw the gel up, so that you should get a U-shaped cavity.
I got the idea when looking at this web page, where the company specifically tries to avoid that effect:
https://ibidi.com/multiwell-plates/23--plate-96-well-3d.html
Best
Steffen
Am 01.06.2023 um 13:49 schrieb Ann Wheeler:
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Dear All
Does anyone know of a manufacturer, or other solution of U bottomed plates suitable for microscopy. Essentially we need the U bottom equivalent of the Black walled 'optically bottomed' 96 and 384 well plates used for High Content screening. Alternatively has anyone come up with a smart solution, which isn't colouring in the walls of the wells with a black permanent marker (which will work but isn't really ideal for organoid screening).
The organoids can't be moved to a flat bottomed plate. Probably like others we are looking at reporter gene assays and we are expecting to see differential expression of fluorescently tagged genes both spatially and temporally. Further the organoids are sufficiently large we don't need super high magnification.
Thanks
Ann
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-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Jakub Chojnacki
Sent: 01 June 2023 12:34
To: [log in to unmask]
Subject: Re: Leica tauSTED vs. Abberior STEDYCON
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Hello,
I thought to add my two cents to this thread, having previously used all the Abberior and Leica scopes.
Firstly, I believe that Stellaris 8 and STEDYCON are in different classes (evident just by the price tag alone), with Stellaris 8 being a flagship product comparable rather to Abberior Infinity or Facility Line.
In my opinion, STEDYCON is a great machine (it's amazing what they crammed into its PC case-sized box), if you need a more compact setup with a bit more limited features than flagship scopes. It works great as a basic 2D STED microscope and is a competent confocal in its own right (I know of places that use it almost almost solely as confocal). Moreover, it runs on a different software (built from ground up) than Infinity or Facility scopes, which I, and the users I demoed it to, found very easy to use.
Hence, it is a great choice for STED scope if you perhaps do not have the cash for Stellaris, need something more compact or if you already have an existing microscope body that you would want to upgrade to confocal/STED.
Hope this helps.
Regards,
Jakub
*---Jakub Chojnacki, PhDHead of IGTP Microscopy Facility* Germans Trias i Pujol Research Institute (IGTP) Campus Can Ruti Carretera de Can Ruti, Camí de les Escoles s/n
08916 Badalona, Barcelona, Spain
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On Wed, 17 May 2023 at 00:25, Michael Kirber <[log in to unmask]> wrote:
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We have an Abberior Facility STED system and are extremely pleased
with the performance and the excellent support from Abberior. We also
have an older Leica SP5 which is an excellent general purpose
confocal, and have had good support from Leica. I think that the
Abberior Facility line has several advantages over the Leica STED but
I am not totally familiar with that product. 1) It has the spatial
light modulators which allow 3D STED without deconvolution or other
processing. 2) It has all pulsed lasers for excitation and depletion
with the exception of the 405nm one. This not only expands the
possibilities for STED experiments but also allows for more FLIM
options either in STED mode or regular confocal mode. 3) The system
autoaligns itself in a very easy user-friendly procedure. Although
the software is well designed and user friendly, I would not choose
this system as a general purpose confocal microscope that you could turn students loose on without significant training.
The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. Is e buidheann carthannais a th’ ann an Oilthigh Dhùn Èideann, clàraichte an Alba, àireamh clàraidh SC005336.
--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
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Head of the Core Facility Bioimaging
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