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Hi Doug,
Since a water pipe burst in the ceiling above the confocal in the Ophthalmology dept,, we are providing confocal service until their system is repaired.  I just spoke with someone imaging a whole mouse retina, he was using a 60X/1.35 oil immersion lens with a retina mounted in Vectashield,and  routinely images to ~150 um depth.  I can't say whether that is the optimal preparation, but its a leading lab in the field.   Vectashield is essentially glycerol, so that may be a good place to start.    
Regards,
Glen
Glen MacDonald
	Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
	Cellular Morphology Core
Center on Human Development and Disability
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
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On Jun 19, 2013, at 2:29 PM, "Cromey, Douglas W - (dcromey)" <[log in to unmask]> wrote:

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> 
> I have a lab that is starting a project that will involve confocal imaging of whole mount retinas (small rodents).  We were able to image to-pro3 labeled nuclei through several layers to a depth of approximately 100um using a 63x water immersion objective.
> 
> Is there anyone that does something similar that would be willing to offer up some sample prep tips?
> 
> I recognize that spherical aberration becomes a significant issue when imaging that deep.  Any suggestions for possible clearing techniques (hopefully something not too complicated)?
> 
> Doug
> 
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
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