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Dear Elaine,

We have developed programs for alignment and composing
neighboring (overlapping) confocal stacks and confocal stacks in
successive physical sections - see Karen et al., Microsc Res Tech
62: 415-422, 2003. The programs (running under MS Windows),
especially useful for Bio-Rad PIC files, are available for free -
contact me or Petr Karen ([log in to unmask]).

Lucie
_______________________________________________________
Dr. Lucie Kubinova
Chair, Department of Biomathematics
Institute of Physiology
Videnska 1083
CZ-14220 Prague 4
Czech Republic
Phone: (420) 29644 2314
Fax: (420) 29644 2488
E-mail: [log in to unmask]

> Has anyone taken a series of confocal stacks of a specimen that is
> bigger than the field of view and montaged the stacks to put the
> whole specimen together?
>
> If so, what techniques did you use to make sure that the sections
> were at the right level and what program did you use?
>
> Thanks
> Elaine
>
> --
> Dr. Elaine Humphrey
> Director, BioImaging Facility
> President, Microscopy Society of Canada
> University of British Columbia
> 6270 University Blvd, mail-stop Botany
> Vancouver, BC
> CANADA, V6T 1Z4
> Phone: 604-822-3354
> FAX:   604-822-6089
> e-mail: [log in to unmask]
> website: www.emlab.ubc.ca