In message Don-Carlos Abrams writes:
> I am intesrted in measuring the nuclear to cytoplasmic volume density of
> cells f
> ixed in 95% alcohol.   I would therefore be grateful for any information
> regardi
> ng nuclear and cytoplasmic markers that are suitable for confocal imaging.
 
--What tissue?  In neurons, ethidium bromide marks the cytoplasm well.  In many
other cells, it's a nuclear stain.  It can be excited well at 488 nm, 514 nm,
and 543 nm; 568 nm will also work if you want to avoid exciting fluorescein.
Its emission is orange/red and should be distinct from fluorescein, as long as
you monitor fluorescein emission at wavelengths shorter than about 550 nm.
 
Good Luck!
 
Martin Wessendorf