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Hi Ali 

I’m not sure this is the same issue but we found that many functions in imaris move things to the nearest pixel so basically don’t work at sub pixel resolution.  Totally invalidates any particle tracking in imaris! 

Ian

Sent from my iPhone

> On 14 Mar 2018, at 14:24, Dun, Alison <[log in to unmask]> wrote:
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Hi
> 
> Have a facility user who is trying to make measurements using Imaris (version 7.3.1) and having some difficulties. Ideally she would like to use the slice section but cannot see how to do this in the software - maybe it is an upgrade we don’t have? Is there a way to do it in 3D consistently? When you move the navigator tool the line is not where you attempted to put it! Is there another software that is better to use?
> 
> Any help would be much appreciated!
> Ali
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