CONFOCALMICROSCOPY Archives

February 2013

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From:
Talley Lambert <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 27 Feb 2013 19:26:33 -0600
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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Steffen,

Yeah, the whole app is a javascript app, so, as you have found, disabling 
javascript will completely break the app.  Sorry, I wouldn't be able to do it as a 
web-app without using javascript (and I don't know much about making native 
apps, java applets, or flash-based apps).  But I totally understand the desire for 
security, so here's all the scripts and what they do (flash is not required by the 
way):

Required:

iscopecalc.com/js/calc.js
this is the main script file that I wrote that runs the calculator and controls all of 
the interactions and calculations.

http://ajax.googleapis.com/ajax/libs/jquery/1/jquery.js
this is the jQuery javascript library (being served from a google content delivery 
network), used on about half of the websites on the internet, including google, 
amazon, etc....  it is basically just a way for me to more easily interact with the 
HTML document.  you can learn more at http://jquery.com

http://code.jquery.com/mobile/1.3.0/jquery.mobile-1.3.0.min.js
this is the jQuery mobile library.  it is required for forming the interface of the 
calculator and is also required. you can learn more at http://jquerymobile.com

Optional: 

mathjax.org
this script used to render the equations listed on the equations page.  you can 
disable it but the equations will look like mumbo-jumbo...

google-analytics.com
basic usage statistics such as browser type and such are indeed sent to google 
analytics.  however, this is totally optional and you can either block this with 
noscript, or, if you like you can use the URL www.iscopecalc.com/?analytics=off  
which will not send any information to google analytics.

That said, using Firefox 19 with NoScript installed, I am able to successfully use 
the calculator with both mathjax and google analytics disabled (as long as 
jquery, jquerymobile, and iscopecalc are allowed).  So, if you are still unable to 
use it with Firefox after enabling those three required scripts, please email me 
directly, as I'd like to figure out what the problem might be.

With regards to laser scanning calculations... I'm not exactly sure what the 
purpose of the calculator would be if you had the freedom that a LSM provides 
and can set the pixel and pinhole size as you desire.  Are there calculations that 
are not done automatically for you that you'd like to see included?  If you can 
just set pinhole to 1 AU and chose an arbitrary pixel size appropriate for nyquist 
sampling... what is the need for a calculator?

thanks for writing!
-Talley




On Tue, 26 Feb 2013 17:50:25 +0100, Steffen Dietzel <[log in to unmask]> 
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Talley,
>
>my main criticism is that it is not working. I use Firefox 18, for
>saftey reasons with NoScript and Flashblocker.
>
>Clearly I have to allow scripts from iscopecalc.com but why is it
>required to allow scripts from three more web sites including
>google-analytics? I don't like that. Even if I allow them all and allow
>Flash "for this Website" it still doesn't work. For cases like that I
>have an "open" Win Explorer and there it seems to be fine.
>Other than that, since I don't have a spinning disk but a point scanner,
>It would be nice to be able to freely define pixel/voxel size and
>pinhole size.
>
>Cheers
>
>Steffen
>
>
>
>On 21.02.2013 20:49, Talley Lambert wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi all,
>>
>> I've been working on a microscope calculator web-app.  At the risk of 
presenting something well below
>> the expertise level of the average reader here, I'd like to share it with you!  
At the very least, I'm
>> hoping it might be of educational use for students of microscopy… if not also 
of practical use for
>> microscope users.
>>
>> It is primarily designed as a widefield/spinning disk calculator and should be 
useful in assessing
>> sampling rate and/or confocality in a variety of optical setups.  The 
calculator takes a variety of inputs
>> related to your optical setup, and pumps out values for things like lateral 
resolution, axial resolution,
>> sampling rate, field of view, amount of camera chip used, etc…  It includes 
the ability to calculate Airy
>> Units and confocality with adjustable pinhole systems such as the Yokogawa 
W1, or the Borealis mod
>> from Spectral.  It has chip parameters for many of the more popular 
cameras so you can quickly
>> determine the appropriate settings (with binning or optical relays) to achieve 
Nyquist sampling with your
>> given camera. There is also a graphical representation of a diffraction 
limited point source for quick
>> visualization of the current settings.
>>
>> The calculator is designed to be easily viewed on a desktop browser (though 
support for Internet
>> Explorer 8 or earlier is weak) or as an app on a mobile device such as an 
iPhone or iPad.  You can also
>> save configurations for later recall (which might be useful in a core setting 
with a number of fixed
>> configurations).
>>
>> Here is the link:
>>
>> http://iscopecalc.com
>>
>> If you like it, please use it and share it!  I would LOVE to hear your thoughts 
and ideas for improvement
>> or complaints about bugs or inaccuracies!  Feel free to contact me directly.
>>
>> Thanks!
>> -Talley
>>
>> ~~~~~~~~~~~
>> Talley Lambert, PhD
>> Dept. VCAPP
>> Washington State University
>> tlambert [at] vetmed.wsu.edu
>>
>
>
>--
>------------------------------------------------------------
>Steffen Dietzel, PD Dr. rer. nat
>Ludwig-Maximilians-Universität München
>Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
>Head of light microscopy
>
>Mail room:
>Marchioninistr. 15, D-81377 München
>
>Building location:
>Marchioninistr. 27,  München-Großhadern

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