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Hi Debora,

Could you clarify if you are actually power limited?  If this is something
like a 4 watt Chameleon and you are doing tissue imaging at 0.05 watts
power, than most of the discussion is irrelevant.  A simple 50/50
beamsplitter from thorlabs will combine your laser with no effort over the
entire tuning range while still giving you 40 times more power than you
actually need.

Mike



On Thu, Nov 13, 2014 at 12:08 PM, Debora Keller <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> Thanks a lot for all the input - I have now quite some food for thoughts!
>
> I was not aware of the polarization issues, but will keep this in mind
> (though the impact
> on the final image seems to be dependant on the structure observed).
>
> At least now I have a couple of arguments/points to discuss with Leica and
> decide what is
> doable with our setups.
> Cheers
>
> Debbi
>
> PS: obviously, any additional comments will still be read with high
> interest!
>
> Debora Keller, PhD
>
> FILM - Facility for Imaging by Light Microscopy
> - Super-Resolution Microscopy Specialist -
> Sir Alexander Fleming Building, desk 408
> Imperial College London / South Kensington
> Exhibition Road
> London SW7 2AZ, UK
> Phone: +44 (0)207 594 9793
> Mobile: + 44(0) 7760 256 889
> E-mail:  [log in to unmask]
> Website: http://imperial.ac.uk/imagingfacility
>