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Well, I'm not sure about the 510, but on our older (2000) Leica SP2 there
are ports for UV and IR as well as visible lasers.  They follow slightly
different light paths through the scan head.  I assume you can have all
three, but I imagine you'd need to use different objectives, particularly
when using UV or IR, for optimal results.  2-photon is trickier, I guess.

We recently added a 405 laser to our SP2 - required the service engineers
out for a day to replace optics between 405 laser and objectives, and
calibrate.  A very nice addition.
cheers,
Rosemary

Rosemary White                    [log in to unmask]
CSIRO Plant Industry            ph.     02-6246 5475
GPO Box 1600                        mob.  0402 835 973
Canberra, ACT 2601             fax.     02-6246 5334
Australia


> From: Dan Szymanski <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Date: Wed, 9 Nov 2005 14:57:14 -0500
> To: [log in to unmask]
> Subject: Re: 405 and infrared on same LSM 510
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Ok, sounds like fun I think....
> 
> 
> 
> On Nov 9, 2005, at 2:01 PM, John Runions wrote:
> 
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> 
>> Is what Kate wrote below true?  I would really like an LSM510 with
>> 405nm and multiphoton capabilities.  John.
>> 
>> Kate Luby-Phelps wrote:
>> 
>>> I don't think you can have both 405 and IR on the 510. At least that
>>> is what we were told. We went with the IR because we wanted to do 2P.
>>> We are using 2P to excite DAPI at 730 nm.
>>> 
>>> Kate
>>> 
>>> 
> Dan Szymanski
> 226 WSLR
> 494-8092
>