***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Hi Cynthia Did the problem happen slowly or gradually? Do you see a difference between when you just turn on the camera and if you leave it on for a few hours before imaging? Is the whole image blurry and the dark background everywhere or do you have variations from one area of the image to another? There could be condensation on the camera chip. This would explain the blurriness and change of background colour. If this is the case, keeping the camera on for a few hours should improve both blurriness and background colour because the camera heats up and the water evaporates. Unfortunately, the problem comes back when the camera cools down and leaving the camera on all the time is only a temporary solution. If this is the case, the camera needs to be serviced and it should then work as normal. Med vanliga hälsningar/Best regards Sylvie Le Guyader | PhD Live Cell Imaging core facility manager Bionut | Karolinska Institutet Hälsovägen 7C | 14157 Huddinge +46 73 733 5008 [log in to unmask]<mailto:[log in to unmask]> | LCI website<https://ki.se/en/bionut/live-cell-imaging-core-facility-lci> | LCI blog<http://microscopykarolinska.se/> Karolinska Institutet – a medical university ________________________________ From: Confocal Microscopy List <[log in to unmask]> on behalf of Cynthia Nwamaka Ikeji <[log in to unmask]> Sent: Tuesday, September 26, 2023 2:44:01 PM To: [log in to unmask] <[log in to unmask]> Subject: Re: Microscope camera [You don't often get email from [log in to unmask] Learn why this is important at https://aka.ms/LearnAboutSenderIdentification ] ***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FSUBED1%3Dconfocalmicroscopy%26A%3D1&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267024472%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=A4Dop16p%2BfGHeJrEJS5svx%2B0gqImijjKEo52CgyzPsQ%3D&reserved=0<https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1> Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267024472%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xMPl4Eq04P4eA1sfXHoAZqu334%2FRK2hWiaklKBHx%2BjY%3D&reserved=0<http://www.imgur.com/> and include the link in your posting. ***** Dear Sylvie, Thank you for reaching out to me. I presume it's the camera because viewing tissues via the microscope eyepiece gives a vivid image but then, when the images are captured it doesn't come out well. The color is different, background is dull, and images are not sharp enough. The camera model is RisingCam C3CMOS10000KPA. Best regards Cynthia On Tue 26 Sep 2023, 9:16 a.m. Sylvie Le Guyader, < [log in to unmask]> wrote: > ***** > To join or leave the confocal microscopy listserv or to change your email > address, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FSUBED1%3Dconfocalmicroscopy%26A%3D1&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267024472%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=A4Dop16p%2BfGHeJrEJS5svx%2B0gqImijjKEo52CgyzPsQ%3D&reserved=0<https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1> > Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267024472%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xMPl4Eq04P4eA1sfXHoAZqu334%2FRK2hWiaklKBHx%2BjY%3D&reserved=0<http://www.imgur.com/> and include the link in your posting. > ***** > > Dear Cynthia > > How do you know that the problem comes from the camera? What camera do you > have? > > Med vanliga hälsningar/Best regards > > Sylvie Le Guyader | PhD > Live Cell Imaging core facility manager > Bionut | Karolinska Institutet > Hälsovägen 7C | 14157 Huddinge > +46 73 733 5008 > [log in to unmask] | LCI website | LCI blog > > Karolinska Institutet – a medical university > > -----Original Message----- > From: Confocal Microscopy List <[log in to unmask]> On > Behalf Of Cynthia Nwamaka Ikeji > Sent: Tuesday, September 26, 2023 9:41 AM > To: [log in to unmask] > Subject: Microscope camera > > [Some people who received this message don't often get email from > [log in to unmask] Learn why this is important at > https://aka.ms/LearnAboutSenderIdentification ] > > ***** > To join or leave the confocal microscopy listserv or to change your email > address, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FSUBED1%3Dconfocalmicroscopy%26A%3D1&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267180754%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YJ6dxSlY6%2F7%2F2ekN9MoSEYUooH9xr45LcIq%2FeoVyN%2BU%3D&reserved=0<https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1> > Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267180754%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zPkun1U5VEvDwcGhSJ02g4PLYGwuBNHS7sVgtikkIoc%3D&reserved=0<http://www.imgur.com/> and include the link in your posting. > ***** > > Dear list members, > Greetings to you all. Please I am in need of a good camera for our Olympus > light microscope in my laboratory as images captured with our current > camera are not sharp enough and distinctive. Any advice on which one to get > and how I can go about it? If there's one that's not being used and > wouldn't mind to give out please let me know. I'm from University of > Ibadan, Nigeria. Thanks. > > Regards, > Cynthia > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI > kommer att behandla dina personuppgifter. Här finns information om hur KI > behandlar personuppgifter< > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fki.se%2Fmedarbetare%2Fintegritetsskyddspolicy&data=05%7C01%7Csylvie.le.guyader%40KI.SE%7C0ed40b2d0685475f4a5e08dbbe8eeaf8%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C638313293267180754%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=XG8%2F1LDoDpVdwvGEND3bnSopBOnt1K74fGKDBL04Vfw%3D&reserved=0><https://ki.se/medarbetare/integritetsskyddspolicy>. > > > Sending email to Karolinska Institutet (KI) will result in KI processing > your personal data. You can read more about KI’s processing of personal > data here<https://ki.se/en/staff/data-protection-policy>. >
