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March 2008

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Subject:
Advice on deconvolution software for widefield microscope
From:
Pedro Camello <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 31 Mar 2008 19:07:14 -0400
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Hi all

We´re considering purchase a deconvolution package to acquire images in a
TE2000 Nikon with a Orca-II ER camera. Deconvol would be oriented to resolve
subcellular structures at high magnification and tissue structures at low
(x10-x20) mag (if that´s possible). I need opinions regarding:

1. Best software
2. Best z control for the TE2000 (objectives or stage?)

I would also to know whether Z motorization is necessary only occasionally,
to calculate PSF, or is a used in each deconv-aimed acquisition.

Thanks in advance

  Pedro

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