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June 2012

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Subject:
From:
Craig Brideau <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 21 Jun 2012 10:27:33 -0600
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Our lab has had some success with the Mattek brand 96 well
coverslip-bottomed plates.

Craig


On Thu, Jun 21, 2012 at 10:08 AM, Julio Vazquez <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Our customers had good success with Greiner Bio-One coverglass bottom
> plates, although I was not able to find them on their online catalog in a
> recent search.
>
> hhttp://us.gbo.com/bioscience/categories/
>
> Their Polysterene plates Cat # 655083/655086 are also good.
>
>
> I've had good recommendations for Ibidi plates and Willco wells plates.
> They are supposed to be good optically, and cells are reported to grow well
> on them:
>
> http://www.biophysics.com/pages/products/IBIDI/ibidi.html
>
> http://www.ibidi.de/products/index.html
>
> http://www.willcowells.com/
>
>
> Although not coverglass bottom, optical plates from BD and Nunc have also
> given us good results. They do work well with lenses such as 20x/0.8
> PlanApo, but we did not use them with higher NA immersion lenses; BD used
> to have also glass bottom plates (#357311); I'm not sure they still do. The
> BD Thin Bottom series (#353219) are "optical grade" plastic 190 microns
> thick. BD claims they are suitable for use with high NA objectives.
> Assuming the refractive index is right, oil immersion lenses shoudl not
> care too much about a 20 micron difference in thickness.
>
> Nunc Optiplate: Nunc Cat # 165306; Sigma-Aldrich Cat # P8866; Fisher
> Scientific catalog # 12-566-35 and 12-566-37
>
> (Nunc plates now sold by Thermo-Fisher)
>
> Falcon BD BioCoat: 96-well clear bottom and 384 well thin bottom, BD Cat #
> 353219 (Fisher cat # 08-772-225)
>
> Falcon BD BioCoat 384-well: BD bioscience cat 354667 (collagen coated) and
> others
>
> BD Falcon glass bottom plates: # 357311 (96-well) / 357312 (384-well)
>
> These plates come with different coatings, and therefore may have
> different catalog numbers. Just make sure to read the full specs before
> ordering.
>
>
>
> --
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> http://www.fhcrc.org/
>
>
>
> On Jun 21, 2012, at 2:02 AM, Adrian Smith wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi all,
> >
> > We have a user who is after some 96well plates for hi-res imaging, ie
> coverslip thickness (glass?).
> >
> > Any comments/recommendations would be appreciated.
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
>

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