Dear,

We are using Congored as specific cellulose binding stain. I use both the
488 and 514 line of the Argon Ion laser for the excitation, and detect the
signal with 585LP.
A good reference about this stain is  Verbelen and Stickens, In vivo
determination of fibril orientation in plant cell walls with
polarization CSLM (1995); Journal of Microscopy, 117, 1,6.

Good luck!

Nathalie Van Bruaene
Lab. Biochemistry and Molecular Cytology
Coupure Links 653
9000 Gent
Tel: 09/264 59 70
Fax: 09/264 62 19

http://biochema.rug.ac.be/Nathalie/Nathalie.html


On Thu, 8 Jul 1999, Robert J. Palmer Jr. wrote:

> I have a colleague who would like to use Congo Red as a polysaccharide
> stain in preps to be examined with a laser confocal.  Does anyone have
> experience with excitation/emission characteristics of this stain (which I
> presume to be a chromophore not a patently "fluorescent" dye)?  I know the
> confocal unit has UV, FITC and TRITC excitation wavelengths.  It may also
> have reflectance scanning, which I presume would work if no other dyes were
> in the mix.
> Rob Palmer
> CEB/UT
>