OK, in order to be exhaustive on cytosolic staining of nucleic acid dyes, we
should note that RNase resistant cytosolic staining in cultured cells may
indicate mycoplasma contamination as well. In that case, you may wish to
check your cells by PCR.


--
Zsolt Lenkei, MD, PhD
Laboratoire de Neurobiologie
ESPCI - CNRS UMR 7637
10, rue Vauquelin
75005 Paris, France
Tel: (33-1) 40 79 51 84
Fax:  (33-1) 40 79 47 57



> From: jordan <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Newsgroups: bit.listserv.confocal
> Date: Fri, 9 Feb 2001 12:02:18 +0000
> To: [log in to unmask]
> Subject: Re: Nuclear fluorochrome
>
> If you wish to completely remove all the cytosolic staining, after the
> RNase digestion, incubate in 2 Molar HCL for 20 minutes. Some protocols
> suggest diluting the stain in 0.1 N HCL as well.
>
> Peter Jordan
> ICRF
> London
>