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Date: | Tue, 10 Aug 2010 15:58:36 -0400 |
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Dear Listers,
I'm doing a set of experiments which requires high magnification (60x
objective + x36 pan zoom) on our confocal Olympus FV1000. the
microscope is placed on the air table, which do not touch any wall or
tables around.
Imaging was going well, but at some point I started to see a zigzag
pattern in the scanning, similar to that one gets when the air table
is lucking nitrogen. This seems not to be a problem of the scanner
itself, using a separate scanning unit delivers the same zigzag.
The lab is on the 7th floor, but this shouldn't be the case as for
about a month the imaging was going well.
I'm wondering what could be a source of this problem in a typical lab,
and what could be done to overcome it.
Thank you in advance,
Vladimir Ghukasyan
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