Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sound like a possible spherical aberration artifact to me. I think the
software assumes a symmetrical PSF if this is not the case you will get
strange one sided artifacts.

Regards Mark Cannell

Robert Kolb wrote:

> I have recently been deconvolving images of an epithelial monolayer
> stained with Wheat Germ Agglutinin, in which the mounting media is
> Prolong Antifade from Molecular Probes. My problem is I appear to be
> getting some weird diffraction patterns (X like patterns) in the
> planes above the apical side of the cells. The diffraction patterns do
> not seem to correspond in anyway to any of the cell membrane
> structures. I am using Autoquant imaging software for the
> deconvolution. If someone would like to see the patterns I am speaking
> of I can send a copy of the image. Any insight would be greatly
> appreciated.
>
> Thanks,
>
> Robert J. Kolb, Ph.D.
>
> CaseWestern ReserveUniversity
>
> Physio. & Biophys. Dept.
>
> Cleveland,OH44106-4970
>
> 216.368.5533
>