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I think yould can ga for advise to SVI.nl fot deconvolution questions,
mayve they can give you an advise!
Good luck,

Tineke Vendrig


>
> 2012/11/8 Kate Luby-Phelps <[log in to unmask]>
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> I have a user who has unavoidable brightly fluorescent blobs in his field
>> of view
>> that interfere with deconvolution of the less fluorescent structures. I
>> just found
>> out he has been masking them out before deconvolving by making isosurface
>> objects and setting the voxels inside the object to zero. From what I
>> understand
>> about deconvolution, this seems like a bad idea because it introduces (a)
>> void(s)
>> with sharp edges in the dataset as well as clipping out information that
>> likely
>> came from regions of the specimen outside the blob. I think he should
>> crop the z
>> stack to exclude the blobs and then deconvolve but I don't know how
>> important
>> this issue actually is. Can anyone advise me on this?
>>
>> Thanks
>>
>> Kate Luby-Phelps
>>
>
>