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Great timing for this thread.

Basically, 512 still seems to be preferred for live cells spinning disk
imaging because of speed. 
Top of the line seem to be the new Cascade II by Roper photometrics, the new
image EM by Hamamatsu, the Rolera  by Qimaging and the iXon by Andor.
Does any one stand out from the lot for a reason or another, like chip brand
or cooling mechanism for ex. ?

Thanks

Marc


 

-----Message d'origine-----
De : Michael Cammer [mailto:[log in to unmask]] 
Envoyé : 11 avril 2007 17:54
À : [log in to unmask]
Objet : Re: EMCCD question

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Dark noise at -100 degrees C.  This is what I was told, not what I 
measured personally.

BTW, Andor helped us with the software and the camera is now imaging 
with IPLab.

-mc


At 05:09 PM 04/11/07, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi Mike- Can you clarify what the test was and what 2-3x better
>means? Dave
>
>Dr. David Knecht
>Department of Molecular and Cell Biology
>Co-head Flow Cytometry and Confocal Microscopy Facility
>U-3125
>91 N. Eagleville Rd.
>University of Connecticut
>Storrs, CT 06269
>860-486-2200
>860-486-4331 (fax)
>
>
>On Apr 11, 2007, at 10:37 AM, Michael Cammer wrote:
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>>
>>>2) Roper and Andor offer cameras with all three chips, Hamamatsu
>>>only with (a) and (b).  Do you see any difference between the
>>>different companies (besides the specifications/marketing  slogans).
>>
>>
>>A postdoc here just compared the 512 versions.   The Hamamatsu
>>didn't work.  The Andor is slightly faster streaming.  I didn't
>>think there was much of a difference between the image quality of
>>the Cascade and the Andor, but the postdoc claimed that when cooled
>>to -100 degrees, the Andor was 2X to 3X better.  We purchased the
>>Andor, but might return it because we can't get it to work with IPLab.
>>
>>-mc
>>______________________________________________________________________
______
>>Michael Cammer   Analytical Imaging Facility   Albert Einstein
>>Coll. of Med.
>>URL:  http://www.aecom.yu.edu/aif/

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
URL:  http://www.aecom.yu.edu/aif/