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Hi all,

Would like to ask anyone who has used LabTek II's Chambered Coverglass and
Chamber Slide.

I read from the FAQ on their website, that the Chamber Slide's upper
structure is made of polystyrene, which is not compatible with many fixation
reagents; and thus, it is designed for upper structure and gasket removal
before fixing, staining and coverslipping.

However, i found from their comparison chart that the upper structure of
their Chambered Coverglass is also made of polystyrene, BUT the upper
structure is non-removable. Why is it so? Does this mean that fluorescence
staining has to be done elsewhere prior to transfer into the Chambered
Coverglass? And are the two products suitable for fluorescence work with
inverted microscope? I have the impression that Chambered Coverglass is
great for inverted microscope, and Chamber Slide is for upright microscope.

Please advise. Thank you!

Cheers,
Rachael Lim