>
> Has there been any validation experiment done to determine if there is
> compression of thick tissue specimens (100-200 microns) during confocal
> imaging with oil-immersion lenses (40/60/100X)?  I am imaging spinal cord
> sections of 150 microns, and the neurons in them are distributed all across
> the thickness of a section.  I am interested in determining if the estimates
> of thickness that I get for neurons on one side of the section are affected
> by any compression of the tissue by the advancing objective during data
> collection.
>
> Any input is greatly appreciated.
>
I instruct anybody who uses our confocal to put a spacer between the
cover slip and slide so that any cells or tissue will not undergo the
force of said advance of objective.
-MC
 
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