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Indeed - I'd like to second that question.

Right now I'm using 850nm for mTurq2. This may be suboptimal, though.

Well: I should probably just test it.

Tobias


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cameron, Lisa
Sent: Friday, September 20, 2013 15:40
To: [log in to unmask]
Subject: 2-photon excitation wavelength recommendations for mTurq2 and clover

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Hello -

Have people tested and found optimal 2-photon laser excitation wavelengths for some of the newer fluorophores like mTurquoise2 and Clover? 

I'd be interested to hear what people have tried / found.

Our specific application is FLIM-FRET.
We do the measurements with a Becker & Hickl time domain system on a Zeiss 710 with 2p laser.

Thank you for any information -
Lisa

---------------------------------------
Lisa Cameron, Ph.D. 
Director of Confocal and Light Microscopy Core Dana Farber Cancer Institute
450 Brookline Ave.; JF 621
Boston, MA 02215
http://microscopy.dfci.harvard.edu


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