> From:          Mikael Gustafsson <[log in to unmask]>
> Subject:       Capillaries
 
> I4m setting up an endothelial capillary culture system for
> microscopy according to Cooke 1993. Does any one know where to find
> flat glass capillaries with an optical quality suitable for high res
> microscopy ? Any suggestions are welcome. Does any one have
> experience of such culture systems ?
>
 
Amazing ! I was just looking at a paper where an optical "chamber"
and microfluorometry was used to measure mitochondrial calcium when
Mikael's mail came in.  I am also looking for alternatives so please
post all possibilities.
 
Reference:
Regulation of cardiac mitochondrial calcium by average extramitchondrial
calcium. Leisey et al.  Am J Physiol 265:H1203-1208, 1993.
 
Square glass capillaries, 0.9 x 0.9mm internally and with 0.18mm wall
thickness.  Apparently suitable for measuring Fura signals with a
Nikon Fluor 40x obj. lens.
 
 Available from Wilmad Glass Co, Rte 40 & Oak Rd, Buena,
NJ, NJ 08310,USA. Tel (609) 697-3000; Fax (609) 697-0536.....I got
the address and tel from an old directory, so they may not all be
correct - please check !
 
Ian Harper
 
 
*********************************************************
Dr Ian Harper
Experimental Biology Programme
Medical Research Council
PO Box 19070                         Tel: 027-21-938 0347
Tygerberg 7505                       Fax: 027-21-938 0456
South Africa            Internet: [log in to unmask]
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